Yield will gradually decrease as length of oligo increases. Not recommended for oligos longer than 35 mer. Yield and Purity will be lower for sequences with high GC content **RPC is reverse phase purification using a cartridge a substitue for HPLC.ĭepending on oligo sequence and structure Gel purification recommended for oligos above 50 mer and all applications involving cloning and mutagenesis. No further purification required for PCR and sequencing applications. Refer to coupling efficiency table for oligo length dependent purity and yield. Scale of Synthesis & Yield of Unmodified DNA Oligos Oligo Scale of Synthesis and Typical Yield of Unmodified DNA Oligos*ĭepending on oligo sequence and structure. Oligo Size, Scale of Synthesis & Purification GuidelinesĪll Gene Link oligos shorter than 40 mer usually do not require any further purification if the application is for PCR or sequencing.Ĭlick here for RNA Oligos scale of synthesis and yield. Purification is strongly recommended for oligos longer than 50 mer. A 60 mer crude product will contain ~75% full-length oligo and similarly a 100 mer will contain ~60%.
#SNAPGENE PRICES PER BASE FULL#
Gel picture will be mailed if requested.Īll Gene Link oligos shorter than 40 mer do not require further purification if the applicaton is for PCR or sequencing.Ģ0 mer oligo synthesized at a coupling efficiency of 99.5% will contain ~90% full length 20 mer and a mixture of truncated sequences of ~10%.Īs the length of the oligo increases, even at a coupling efficiency of 99.5%, the yield of the full length oligo is reducing. Received before 12 noon EST gel picture may not be provided due to shipping time constraints.
Shipped the same day for next day am or pm delivery. Is the case, order our Same Day Oligo service. Same Day Oligo Service $35.00 per oligo (Must order by 12 PM EST Time)Ĭertain experiments cannot wait for an extra day. Unmodified Oligonucleotides (price per base)* Price List for Unmodified DNA Oligonucleotides
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